ESR8

Host institution: Università Pavia, Italy

ESR8 – Signal processing in cerebellar modules involved in emotional, cognitive and motor control loops

This project will determine how signal processing differs between different parts of the same cerebellar module, in particular between vermal lobules IV-V, VI-VII, VIII, and IX-X. High density multielectrode arrays (HD-MEA – 3-Brain) will be used to characterize spontaneous activity from Purkinje cells, Golgi cells and molecular layer interneurons within each lobule, as well as responses to electrical stimulation of mossy fibre bundles. Advanced techniques for LFP modelling (Diwakar et al, 2011; Casali et al., 2019) will be used to extract information about the spatio-temporal organization of the underlying neuronal circuit activity. The HD-MEA work will be complemented by patch-clamp recordings to provide evidence of the cellular mechanisms unveiled by HD-MEA. Collaboration with Bristol and 3-Brain.

This project will provide new insights into neuronal activity within cerebellar modules and how the signal processing varies between different lobules.

Planned secondments: University of Bristol, Richard Apps/Charlotte Lawrenson, month 22-24, purpose: training in combined optogenetic and electrophysiological approaches to understand cerebellar modules.